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Ni Sepharose(R) 6 fast flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins with the well industrially established properties of the Sepharose(R) fast flow platform.Ni Sepharose(R) 6 fast flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.Ni Sepharose(R) 6 fast flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.Ni Sepharose(R) 6 fast flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range Ni Sepharose(R) 6 fast flow meets industrial demands with security of supply and comprehensive tech
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